蛋白芯片詳細

信號通路磷酸化抗體芯片 > 升級版 信號通路廣篩抗體芯片(CSP100_Plus)

    升級版 信號通路廣篩抗體芯片(CSP100_Plus)

     

           
           信號通路是由從細胞膜、胞漿到細胞核一系列的蛋白分子,通過蛋白磷酸化和蛋白互作等形式串聯起來的蛋白分子信號傳導網絡。在疾病的發生、發展,細胞表型等生物現象的背后,信號通路的調變起著分子層面的支撐作用。信號通路分析是腫瘤學、病理學、免疫學、細胞生物學、神經生物學、藥理學等一系列以機理探索為研究目的的科研工作的必經之路。


            然而,細胞中存在多條信號通路,它們并不孤立存在,會串聯交叉形成的復雜信號網絡(Cross-talk)。該信號網絡在細胞受到胞外刺激后將信號通過級聯放大、分散調節等方式傳入胞內,引起一系列復雜的信號改變,進而產生綜合的細胞應答。面對復雜研究現象,往往會有多條通路會發生調變。如何選擇主要調變的通路?是困擾研究人員的主要難題之一,而傳統的技術如Westen blot等不能解決。

                                            


            升級版 信號通路廣篩抗體芯片(CSP100 Plus)
    是在原腫瘤信號通路磷酸化廣譜篩選抗體芯片(CSP100)的基礎上升級完成的廣篩芯片。該芯片采用三維高分子膜專利技術,在片基上共價結合304種高特異抗體,其中磷酸化抗體157種,非磷酸化抗體147種。一張CSP100 Plus芯片可以同時對16條信號通路進行篩選和比較。這16條信號通路是生物醫學研究中常見也是重要的通路,它們廣泛參與到腫瘤、免疫、心血管、神經系統、損失修復等多種病理生理過程中。因此,CSP100 Plus芯片可以適用于多種疾病與藥物研究模型的信號通路篩選工作。同時,脊椎動物的磷酸化位點具備保守性,芯片上的抗體都是以目標蛋白的物種保守多肽序列為抗原進行設置,所以,CSP100 Plus芯片上的抗體具有一定的物種通用性。

    抗體芯片特點:

     

    • 芯片規格為76 x 25 x 1 mm;

    • 實現16條信號通路全面篩選;

    • 每種抗體設置6次技術重復;

    • 適用于組織、細胞等多類型樣本;

    • 5×106細胞、200 μg總蛋白量即可滿足實驗;

    • 可同時提供磷酸化和蛋白表達兩套數據;

    • 可通用于人、小鼠、大鼠等多類型模式生物檢測。


    芯片檢測列表:

    Pathway Name

    Protein Number

    PI3K-Akt signaling pathway57
    MAPK signaling pathway49
    Ras signaling pathway40
    ErbB signaling pathway39
    Focal adhesion38
    Apoptosis pathway35
    Insulin signaling pathway
    32
    Autophagy29
    mTOR signaling pathway28
    JAK-STAT signaling pathway27
    AMPK signaling pathway25
    Cell cycle20
    p53 signaling pathway18
    NF-kappa B signaling pathway18
    VEGF signaling pathway17
    TGF-beta signaling pathway10
    Others13

    5ec7128355222f1b5ffcb113645f672b.gif詳細列表下載

    抗體芯片原理:

                                        

                                                           

                                            

    抗體芯片文獻:


     

    1.      Gu C, et al. Discovery of the Oncogenic Parp1, a Target of bcr-abl and a Potential Therapeutic, in mir-181a/PPFIA1 Signaling Pathway.  Mol Ther Nucleic Acids. 2019 Feb 8; 16: 1-14. (暨南大學)
    2.      Gong X, et al. 
    Expression of ILK in Renal Stroma Is Essential for Multiple Aspects of Renal Development. Am J Physiol Renal Physiol, 2018.(同濟大學)
    3.      Liu D, et al. 
    C/EBPβ enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation. Nat Commun. 2018, 9(1): 1739. (華中科技大學同濟醫院)
    4.      Lei miao Yin, et al. 
    Transgelin-2 as a therapeutic target for asthmatic pulmonary resistance. Sci Transl Med, 2018, 10(427).(上海中醫藥大學)
    5.      Venkatesh, Humsa S., et al. 
    Targeting neuronal activity-regulated neuroligin-3 dependency in high-grade glioma. Nature, 2017, 549(7673): 533-537.
    6.      Gao Y, et al. 
    Mammalian elongation factor 4 regulates mitochondrial translation essential for spermatogenesis. Natstruct Mol Biol, 2016, 23(5): 441-449. (中國科學院生物物理研究所)

    7.     Jiang HL et al. SSRP1 uppresses TGF-β-Driven Epithelial-to-Mesenchymal Transition and Metastasis in Triple-Negative Breast Cancer by Regulating Mitochondrial Retrograde Signaling, Cancer Res, 2016, 76(4): 952-64.(復旦大學附屬腫瘤醫院)

    8.      Kuang XY et al. The phosphorylation-specific association of STMN1 with GRP78 promotes breast cancer metastasisCancer Lett, 2016, 377(1): 87-96.(復旦大學附屬腫瘤醫院)
    9.       Zhu Y et al. 
    Long non-coding RNA LOC572558 inhibits bladder cancer cell proliferation and tumor growth by regulating the AKT-MDM2-p53 signaling axisCancer Lett, 2016, 04(3).(復旦大學附屬腫瘤醫院)

    10.        Luo L L, Zhao L, Wang Y X, et al. Insulin-like growth factor binding protein-3 is a new predictor of radiosensitivity on esophageal squamous cell carcinoma. Sci Rep-UK, 2015, 5: 17336.  (中山大學附屬腫瘤醫院)

    11.       Zhu Y P, Wan F N, Shen Y J, et al.  Reactive stroma component COL6A1 is upregulated in castration-resistant prostate cancer and promotes tumor growth. Oncotarget, 2015, 6(16): 14488.(復旦大學附屬腫瘤醫院)
    12.      Wang T, Han S, Wu Z, et al. 
     XCR1 promotes cell growth and migration and is correlated with bone metastasis in non-small cell lung cancer. Biochem Bioph Res Co, 2015, 464(2): 635-641. (上海長征醫院)

    13.      Chen P, Huang H, Wu J, et al. Bone marrow stromal cells protect acute myeloid leukemia cells from anti‐CD44 therapy partly through regulating PI3K/Akt-p27(Kip1) axis. Mol Carcinogen, 2015, 54(12): 1678-85. (福建醫科大學附屬協和醫院)

    14.      Wan F, et al. Oxidized low-density lipoprotein is associated with advanced-stage prostate cancer. Tumor Biol, 2015: 1-10. (復旦大學附屬腫瘤醫院)

    15.      Zhu R, et al. pH sensitive nano layered double hydroxides reduce the hematotoxicity and enhance the anticancer efficacy of etoposide on non-small cell lung cancer. Acta Biomater, 2016, 29: 320-32.  (同濟大學)

    16.      Li W, et al.  Withaferin A suppresses the up-regulation of acetyl-coA carboxylase 1 and skin tumor formation in a skin carcinogenesis mouse model. Mol Carcinog, 2015, 55(11): 1739-1746. (河北大學)

    17.      Rao W, et al. OVA66 increases cell growth, invasion and survival via regulation of IGF-1R-MAPK signaling in human cancer cellsCarcinogenesis,2014, 35(7): 1573-1581.(上海交通大學醫學院)

    18.      Jia D, et al. Amplification of MPZL1/PZR promotes tumor cell migration through Src-mediated phosphorylation of cortactin in hepatocellular carcinomaCell Res, 2014, (24): 204-217. (上海仁濟醫院) 

    19.      Xu N et al. Activation of RAW264.7 mouse macrophage cells in vitro through treatment with recombinant ricin toxin-binding subunit B: Involvement of protein tyrosine, NF-kB and JAK-STAT kinase signaling pathways. Int J Mol Med, 2013, 32(3): 729-735.(吉林大學)

    20.      Li F et al. Superoxide Mediates Direct Current Electric Field-Induced Directional Migration of Glioma Cells through the Activation of AKT and ERKPLoS ONE. 2013, 8(4): e61195.(第三軍醫大學)

    21.      Ranzato E et al. Epithelial mesenchymal transition traits in honey-driven keratinocyte wound healing: comparison among different honeysWound Repair Regen. 2012, 20(5): 778-785.

    22.       Zhang YM et al. A novel angiogenesis inhibitor impairs lovo cell survival via targeting against human VEGFR and its signaling pathway of phosphorylationCell Death Dis, 2012, 3: e406.(西安交通大學) 

     

     

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